| The Effect of GM-CSF Supplementation in Culture Medium in the Human IVF Programs. |
|
Won Il Park, Hyuck Chan Kwon, Dong Hoon Kim, Hee Kyoo Kang, Myo Kyung Kim, Hoi Chang Lee, Ji Hak Jung, Myong Seop Lee, Ho Joon Lee |
1Department of OB/GYN, Eulji University School of Medicine, Korea.
2Department of Physiology, Eulji University School of Medicine, Korea.
3Medical Science Institute, Eulji General Hospital, Seoul, Korea. |
|
|
| Abstract |
OBJECTIVE
Granulocyte-macrophage colony stimulating factors known to be secreted in murine and human reproductive tract. The development of human, bovine and murine embryos could be promoted by addition of GM-CSF in culture medium. However, the pregnancy and implantation rate of embryos cultured in GM-CSF have not been evaluated. The aim of this study was to assess the effect of GM-CSF in embryo development, pregnancy and implantation rate. METHODS: A total of 191 IVF cycles were divided into control and GM-CSF supplement group (control =96, GM-CSF=95). The embryos were cultured for three day with or without 2 ng/ml of recombinant human GM-CSF. The quality of embryo, developmental velocity, pregnancy and implantation rates were compared. RESULTS: There was no difference in age, number of gonadotropin ampules used, number of oocytes and fertilization. The number of ICSI cycle was higher in GM-CSF group. In GM-CSF group, G-1 grade embryos were the highest in proportion (56.4%), while G-2 grade embryos were highest (44.3%) in control group. The developmental velocity of embryos were not different between GM-CSF and control group. The pregnancy and implantation rates were significantly higher in GM-CSF group than control (47.4% vs. 33.3%, 17.0% vs. 11.1% respectively). CONCLUSION: By adding GM-CSF in culture medium, the quality of embryo, pregnancy and implantation rate could be improved. |
| Key Words:
GM-CSF; Embryo; IVF |
|
Full text via DOI
Download Citation
