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Korean Journal of Fertility and Sterility 2006;33(1):53-60.
Published online March 1, 2006.
Comparison between Human Follicular Fluid and SpermGrad for Sperm Preparation in Asthenozoospermia.
Youn Kyung Chung, Jung Ryeol Lee, Jeong Hee Moon, Hyun Jun Kim, Sang Hoon Han, Byung Chul Jee, Seung Yup Ku, Chang Suk Suh, Seok Hyun Kim, Young Min Choi, Jung Gu Kim, Shin Yong Moon
1Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul, Korea.
2Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Korea.
3Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University, Seoul, Korea.
Abstract
OBJECTIVE
We tested the usefulness of swim-down technique using human follicular fluid (hFF) in sperm preparation. METHODS: Semen samples were obtained from twelve male partners showing asthenozoospermia (sperm motility < 50%) at the time of routine andrologic evaluation in Seoul National University Bundang Hospital. After dividing into two aliquots, each samples were processed either by swim-down using 100% hFF or density gradient using SpermGrad. Sperm quality was assessed by computer-assisted semen analyzer (CASA). RESULTS: Motility, Rapid motility, VCL (curvilinear velocity), ALH (amplitude of lateral head displacement), and hyperactivated sperms were significantly increased, and LIN (mean linearity) was decreased significantly after sperm preparation in both groups. Motility was significantly higher after swim-down using 100% hFF when compared with density gradient using SpermGrad (81.2+/-4.7 vs. 67.6+/-2.3, p=0.02). The other parameters assessed by CASA were not different between the two methods. CONCLUSION: Swim-down method with 100% hFF may be a useful method in preparation of sperm from asthenozoospermia.
Key Words: Asthenozoospermia; Density gradient; Human follicular fluid; SpermGrad; Swim-down


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