In Vitro Fertilization and Embryo Development with Human Frozen Semen

Ku,  Pyong-Sahm

Original Article

Korean Journal of Fertility and Sterility 1984;11(2):59-67.

Pyong-Sahm Ku

Department of Obstetrics and Gynecology Korea University College of Medicine

냉동보존정자(冷凍保存精子)의 체외수정(體外受精)에 관(關)한 연구(硏究)

구병삼

고려대학교 의과대학 산부인과학교실

Abstract

In vitro fertilization have been performed to know whether the frozen semen has fertilizing ability and can be used clinically. The results of cultured and developed embryos obtained are as follows: 1. The semen was frozen in three media for the good viability. The viability was more than 50% and the motility was also moderate (grade III), 2. As the 33 oocytes were collected from 45 follicles, the oocyte recovery rate was 73.3%. Among them, mature and immature ova were 5% each, and premature ova were 69.7%, When the first polar body was appeared, above ova were inseminated after adequate incubation with activated sperms. 3. The main components of three freezing medium containing egg yolk, glycerol and pyruvate respectively were the best for sperm viability, and Ham's F-10 medium was used for the fertilization and culture of eggs. 4. The results of in vitro fertilization of 33 ova, showed the second polar body developed in 12%, polyspermia in 24%, 1-cell embryo in 21% and 2-cell embryo in 9%. One mature ova developed to blastocyst via 16-cell to 32-cell embryo. The fertilization rate was 66%. 5. Above mentioned results represent that the frozen semen has fertilizing ability and can be used practically in the clinic.