Mouse In Vitro Fertilization

Lim,  YT; Choi,  SH; Kim,  JG; Moon,  SY; Lee,  JY; Chang,  YS

Original Article

Korean Journal of Fertility and Sterility 1984;11(2):51-57.

YT Lim, SH Choi, JG Kim, SY Moon, JY Lee, YS Chang

Department of Obstetrics & Gynecology, College of Medicine, Seoul National University

마우스 난자(卵子)의 체외수정(體外受精)에 관(關)한 연구(硏究)

임용택, 최승헌, 김정구, 문신용, 이진용, 장윤석

서울대학교 의과대학 산부인과학교실

Abstract

The success of human in vitro fertilization (IVF) & embryo transfer (ET) has focused attention on the culture conditions that can provide optimal development of the preimplantation embryo. Studies of in vitro fertilization using mouse have direct implications to human IVF, since similar conditions are used for both species. Mouse IVF as a quality control system for human IVF & ET was studied since Feb., 1984. The results were as follows: 1. Egg retrieval following superovulation in IeR mice was l5.1${\pm}$5.3 eggs ovulated/mouse (Mean${\pm}$ S.D.) 2. In vitro cleavage rate was 61.7% (1146 eggs cleaved/l858 eggs inseminated) and % blastocyst was 42.6%. 3. In comparison with two media of Ham's F-10 and m-KRB, in vitro cleavage rate were 40.9%/63.l% and %blastocyst were 44.3%/61.2% (P<0.05). 4. It was concluded that mouse IVF system has a valuable place in human IVF & ET as a quality-control system and in human reproductive physiology as a research model.