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Korean Journal of Fertility and Sterility 1981;8(1):1-12.
Detection of Proteins from Porcine Follicular Fluid and Their Effect on the Maturation of Mouse Oocytes in vitro
In-Ha Bae1, Sung-Yun Hwang2, Soon-O Chung3, Wan-Kyoo Cho2
1Sungshin Women's College
2Department of Zoology, College of Natural Sciences, Seoul National University
3College of Medicine, Yonsei University
돼지 여포액내(慮胞液內) 단백질(蛋白質)의 검출(檢出)과 배양중(培養中)인 생쥐란자(卵子)의 성숙(成熟)에 미치는 그의 영향에 관하여
배인하1, 황성윤2, 정순오3, 조완규2
1성신여자대학
2서울대학교 자연과학대학 동물학과
3연세대학교의과대학
Abstract
It has been already suggested that specific macromolecules in follicular fluid produced by granulosa cells may play a role in suppressing further meiotic maturation of the oocytes. In general, the search for specific macromolecules in follicular fluid using immunological methods has not been rewarding. These studies were designed, by applying more effective immunological methods than conventionally employed, (l) to identify whether some unknown macromolecules are present in the porcine follicular fluid or not, and (2) to clarify the relationship between the oocytes and the specific macromolecules in the follicular fluid. The results obtained were as follows; (1) porcine follicular fluid contained two specific proteins, which were not present in pig plasma and serum. (2) each of two proteins showed electrophoretically fast alpha-globulin and beta-globulin mobilities. (3) these proteins seemed to have inhibitory effect on the maturation of mouse oocytes in vitro. From these results, it can be assumed that pig follicular fluid contains specific proteins which seem to be intra-follicular inhibitor(s) of oocyte maturation.


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