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Korean Journal of Reproductive Medicine 2009;36(4):301-310.
Published online December 1, 2009.
Comparative Results of Embryo Development and Clinical Pregnancy using Sperm Retrieved from Fresh and Frozen-thawed Testicular Tissue from Patients with Obstructive and Non-obstructive Azoospermia.
Yong Seog Park, Su Jin Choi, Sun Hee Lee, Dong Wook Park, Chun Kyu Lim, Jin Hyun Jun, Mi Kyoung Koong, Chan Woo Park, In Ok Song, Ju Tae Seo
1Laboratory of Reproductive Biology and Infertility, Kwandong University College of Medicine, Korea.
2Department of Obstetrics and Gynecology, Kwandong University College of Medicine, Korea.
3Department of Urology, Cheil General Hospital & Women's Healthcare Center, Kwandong University College of Medicine, Korea.
4Present address; Department of Bio-Medical Laboratory Science, College of Health Science, Eulji University College of Health Science, Sungnam-Si, Korea.
Abstract
OBJECTIVE
To compare the embryonic development and pregnancy results using sperms retrieved from fresh and frozen-thawed testicular tissue in patients with obstructive (OA) and non-obstructive azoospermia (NOA). METHODS: A total two hundred twenty-two cycles of TESE-ICSI were performed in OA and NOA. Sperms were retrieved from fresh and frozen-thawed testicular tissue. ICSI was performed patient's own sperm. Fertilization was assessed 16~18 hrs after ICSI. Embryo development and pregnancy rates were analysed. RESULTS: The fertilization rates were significantly different between OA and NOA patients (75.2% vs. 56.7%, p<0.05), however, embryo development did not differ between the groups (96.9% vs. 98.0%). Likewise, OA and NOA groups had no differences in their clinical pregnancy and delivery rates, 33.9% vs. 36.0% and 28.1% vs. 28.0%, respectively. With regard to sperm retrieved from fresh testicular tissue, fertilization rates were significantly different between the OA and NOA groups (76.4% vs. 52.9%, p<0.05); however, embryo development, clinical pregnancy and delivery rates were not different. For sperm retrieved from thawed testicular tissue, the fertilization rates were significantly different between the two groups (74.7% OA group vs. 65.6% NOA group, p<0.05); however, embryo development, clinical pregnancy and delivery rates were not different. CONCLUSIONS: Embryo development and clinical pregnancy did not differ in patients with obstructive and non-obstructive azoospermia, whether sperm retrieved from fresh and thawed testicular tissue were used, although the fertilization rates were different. Therefore, ICSI with sperm retrieved from fresh and thawed testicular tissue could achieve relevant clinical pregnancy results in patients with azoospermia.
Key Words: Obstructive azoospermia; Non-obstructive azoospermia; Cryopreservation; Testicular sperm; TESE-ICSI; Embryo development


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