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Korean Journal of Fertility and Sterility 2001;28(2):87-94.
Published online June 1, 2001.
Effects of Heparin-Binding Epidermal Growth Factor on the Expression of MMP-9 and ATPase gamma-subunit mRNA in the Mouse Embryo.
Seok Ho Hong, Hee Young Nah, Ji Yun Lee, Jung Hee Kim, Chung Hoon Kim, Hee Dong Chae, Byung Moon Kang, Moon Kyoo Kim
1Department of Biology, College of Natural Sciences, Hanyang University, Korea.
2Department of Obstetrics and Gynecology, College of Medicine, University of Ulsan, Asan Medical Center, Seoul, Korea.
OBJECTIVES: The purpose of this study was to evaluate effects of heparin-binding epidermal growth factor (HB-EGF) on the rate of blastocyst formation and hatching in the mouse embryos and the expression of matrix metalloproteinase-9 (MMP-9) and ATPase g-subunit mRNA. METHODS: Late 2-cell mouse embryos was cultured for 72 hours in HTF medium containing with 1, 10, and 100 ng/ml HB-EGF. The mRNA expression level of MMP-9 and ATPase gamma-subunit was detected by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: The rate of hatching was significantly higher (p<0.05) in group containing with 1 ng/ml HB-EGF than other groups. Also, the rate of hatched blastocyst was significantly higher (p<0.05) in 10 ng/ml. The mRNA expression level of MMP-9 mRNA was not shown any difference among groups, but ATPase g-subunit was higher than other groups. CONCLUSIONS: Taken together these results suggest that HB-EGF has the positive effect to promote the blastocyst formation and hatching process and influences the blastocoel expansion by promoting the ATPase mRNA expression in the mouse embryos.
Key Words: ATPase g-subunit; Blastocoel; HB-EGF; MMP-9; RT-PCR


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