| Effects of Heparin-Binding Epidermal Growth Factor on the Expression of MMP-9 and ATPase gamma-subunit mRNA in the Mouse Embryo. |
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Seok Ho Hong, Hee Young Nah, Ji Yun Lee, Jung Hee Kim, Chung Hoon Kim, Hee Dong Chae, Byung Moon Kang, Moon Kyoo Kim |
1Department of Biology, College of Natural Sciences, Hanyang University, Korea.
2Department of Obstetrics and Gynecology, College of Medicine, University of Ulsan, Asan Medical Center, Seoul, Korea. |
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| Abstract |
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OBJECTIVES: The purpose of this study was to evaluate effects of heparin-binding epidermal growth factor (HB-EGF) on the rate of blastocyst formation and hatching in the mouse embryos and the expression of matrix metalloproteinase-9 (MMP-9) and ATPase g-subunit mRNA. METHODS: Late 2-cell mouse embryos was cultured for 72 hours in HTF medium containing with 1, 10, and 100 ng/ml HB-EGF. The mRNA expression level of MMP-9 and ATPase gamma-subunit was detected by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: The rate of hatching was significantly higher (p<0.05) in group containing with 1 ng/ml HB-EGF than other groups. Also, the rate of hatched blastocyst was significantly higher (p<0.05) in 10 ng/ml. The mRNA expression level of MMP-9 mRNA was not shown any difference among groups, but ATPase g-subunit was higher than other groups. CONCLUSIONS: Taken together these results suggest that HB-EGF has the positive effect to promote the blastocyst formation and hatching process and influences the blastocoel expansion by promoting the ATPase mRNA expression in the mouse embryos. |
| Key Words:
ATPase g-subunit; Blastocoel; HB-EGF; MMP-9; RT-PCR |
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