Expression of Inhibin in the Whole-body gamma-irradiated Mouse Ovary.

Kim,  Sang Soo; Lee,  Chang Joo; Yoon,  Hyun Tae; Yoon,  Yong Dal

Original Article

Korean Journal of Fertility and Sterility 2006;33(1):35-44.
Published online March 1, 2006.

Expression of Inhibin in the Whole-body gamma-irradiated Mouse Ovary.

Sang Soo Kim, Chang Joo Lee, Hyun Tae Yoon, Yong Dal Yoon

¹The Research Institute for Natural Sciences, Hanyang University, Seoul 133-791, Korea.
²Laboratory of Toxicogemonics, Department of Life Science, Hanyang University, Seoul 133-791, Korea.

Abstract

OBJECTIVE
The purposes of the present study were to investigate the effect of gamma-radiation on the expression of inhibin-alpha proteins and genes for inhibin alpha, betaA, and betain the ovary. METHODS: Immature mice were whole-body gamma-irradiated with 25% of a lethal dose. At time 0, 3, 6, 12, and 24 hours after the irradiation,the ovaries were collected and used for immunohistochemistry for inhibin-alpha, and RT_PCR for inhibin-alpha, betaA, and betaB. RESULTS: The expression of the immunoreactive inhibins-alpha was maintained at 12 hours post-irradiation and reduced thereafter. The expression of inhibin-alpha mRNA was significantly increased with the time after the irradiation. However there were no significant changes in the expression of betaA and betaB mRNAs. CONCLUSION: It might be thought that inhibin acts as one of the regulatory factors in the gamma-radiation-induced follicular atresia in mice

Key Words: Mouse ovary; Follicle; Inhibin; Radiation