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Korean Journal of Fertility and Sterility 2006;33(2):115-123.
Published online June 1, 2006.
Role of Integrin, FAK (Focal Adhesion Kinase) and ERK (Extracellular Signal Regulated Kinase) on the Suppressed Cell Proliferation of Endometrial Cancer Cells by GnRH (Gonadotropin-Releasing Hormone).
Jong Rak Choi, Dong Wook Park, Dong Soon Choi, Churl K Min
1Department of Clinical Pathology, Yonsei University, Seoul, Korea.
2Department of Molecular Science and Technology, Ajou University, Suwon, Korea.
Abstract
OBJECTIVE
To investigate new signal transduction cascade through integrin, FAK and ERK in the suppressed cell proliferation by GnRH-I and -II. METHOD: Human endometrial cancer cells (HEC1A) were cultured under the following condition: DMEM/F12 (10% FBS). GnRH-I and -II were treated time (0, 5, 10, 15, 20, 30 min; 100 nM) and dose (10 nM or 100 nM; 20 min) dependent manner according to experimental purposes. Cell proliferation was measured using [3H] thymidine incorporation assay. Immunoblotting was utilized to detect proteins. RESULTS: GnRH-I and -II inhibited proliferation of HEC1A cells and induced expression of integrin beta3. Phosphorylation of FAK and ERK were induced by GnRH-I and -II. CONCLUSION: GnRH inhibited cell proliferation via the expression of integrin and FAK, ERK phosphorylation.
Key Words: GnRH; Integrin; FAK; ERK; Phosphorylation
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