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Korean Journal of Fertility and Sterility 1990;17(1):81-85.
Ultrarapid Freezing of Mouse Morulae
CS Baik1, MD Suh1, JH Lee1, KK Lee2
1Infertility Clinic, Department of Obstetrics & Gynecology, College of Medicine Kyung Hee University
2Developmental Biotechnology Lab., Genetic Engineering Center, KIST
생쥐 상실배의 초급속동결
백청순1, 서병희1, 이재현1, 이경광2
1경희대학교 의과대학 산부인과학교실 불임크릭??
2한국과학기술연구원 유전공학센타 발생공학연구실
Abstract
We cryopreserved mouse morulae by a simple ultra-rapid method of freezing embryos directly in $LN_2$ after holding 2min in a $LN_2$ vapor, and thawed them in $37^{\circ}C$ water bath. The time requirements for permeation and dehydration by 2.0 M glycerol and 0.2 M sucrose before freezing were studied. When the embryos were equilibrated for 10 min, the optimun post-thaw survival was obtained. Embryos those developed normally to blastocyst after in vitro culture for over 24hrs were regarded as survival ones. Two experiments to assess post-thaw survival following predehydration in various mixtures of glycerol and sucrose were also accomplished. When sucrose was held constant (0.2 M) and glycerol concentration varied (1.5-3.5 M), post-thaw survival was best (78.0%) in 3.0 M glycerol. When glycerol was held constant (3.0M) and sucrose concentration varied (0.0-1.0M), optimun post-thaw survival (78.0%) was found in 0.2 M sucrose.
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