| The Effect of Fertilization on Capacitation in vitro ; Inverment of Epididymal Secretions and Preincubation Time |
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JM Kim1, BH Suh1, JH Lee1, KS Chung2 |
1Infertility Clinic, Department of Obstetris and Gynecology, College of Medicine, Kyung Hee University
2College of Animal Husbandry, Kon Kuk University |
| 생쥐 정소상체정자의 전배양시간 및 정소상체추출물의 첨가가 체외수정에 미치는 영향 |
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김재명1, 서병희1, 이재현1, 정길생2 |
1경희대학교 의과대학 산부인과학교실(불임크리??)
2건국대학교 축산대학 축산학과 |
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| Abstract |
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Capacitation of mouse spermatozoa in vitro is brought about by epididymal secretions released into the m-Tgrode medium at the time of sperm collection. Epididymal mouse sperm suspension achived by centrifugation were preincubated for a total of 120min with aliquants being removed at 5, 30 and 120min. By gently centrifugation and resuspending in fresh medium, the fertilizing rate of unwashed 5-and 30-min suspensions was increased such that 30-min washed samples did not differ significiantly from full capacitated, highly fertile 120-min unwashed samples. When epididymal suspension was added fertilization of cumulus intact oocyte was markedly inhibited, although fertilization of zona free oocytes was unaffected. Washing sperm suspensions preincubated in the absence of $Ca^{2+}$ with the subsequent introduction of exogenous $Ca^{2+}$ resulted in a significant increase in fertilization rates over equivalent unwashed samples. |
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