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Korean Journal of Fertility and Sterility 1989;16(2):113-118.
Effects of Calcium and dbcAMP on Sperm Motility in Mouse
Moon-K Kim1, Seung-H Oh1, Myung-C Gye1, Hyun-S Yoon1, Joon-Y Lee2
1Department of Biology, College of Natural Sciences , Hanyang University
2Department of Biology, College of Natural Sciences, Chungbuk University
생쥐 정자의 운동성에 미치는 Calcium 과 dbcAMP 의 영향
김문규1, 오승훈1, 계명찬1, 윤현수1, 이준영2
1한양대학교 자연과학대학 생물학과
2충북대학교 자연과학대학 생물학과
In order to study the effects of $Ca^{++}$ and dbcAMP on sperm motility, cauda epididyal spermatozoa of mouse were incubated in the media containing the various concentrations of $Ca^{++}$ and dbcAMP, and Its motility were observed and analyzed. The rate(%) of the sperm with forward motility which may be fertile, reached at the equilibrium state in 30-60 min. after the beginning of incubation. $Ca^{++}$ requirement for the maintenance of sperm motility obviously appeared in 3hrs. after the beginning of incubation. In the medium containing 0.01mM $Ca^{++}$, the rate of sperm forward motility was highest(30.6-40.6%), whereas in 10mM $Ca^{++}$ medium, the rate was rather reduced because of a severe agglutination phenomenon. On the other hand, 1mM dbcAMP in the medium was significantly effective for the maintenance of sperm forward motility.


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