| Mouse Embryo Culture as Quality Control for Human IVF:Culture Media and Supplements |
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Gy-Soog Lee, Jong-Duk Park, Choon-Khoon Lee, Jong-Duk Kim |
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Department of Obstetrics and Gynecology, College of Medicine, Chonbuk National University |
| 인간 난자의 체외수정을 위한 정도관리로서 생쥐 착상전 배아의 배양에 관한 연구 |
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이기숙, 박종덕, 이춘근, 김종덕 |
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전북대학교 의과대학 산부인과학교실 |
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| Abstract |
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The development of 2-cell mouse embryos to the blastocyst stage in vitro has been used as quality control for the culture media and supplements employed for human in vitro fertilization and embryo transfer(IVF-ET). 2-cell mouse embryos were cultured to the blastocyst stage in SECM, Medium 199-Earle's, Ham's F-10 I , Ham's F-10 II , Hoppe & Pitts, MEM and $HT_6$. The protein supplements contained in media were bovine serum albumine, fetal bovine serum and human fetal cord serum. The results were as follows; 1. The successful development was 81.3% in Medium 199-Earle’s, 91.9% in Ham’s F-10 I and 97.1% in $HT_6$. 2. 2-cell mouse embryos developed properly in all supplements but the best development was particularly noted in $HT_6$ media when HFCS was supplied as protein supplement. |
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