Effects of Calcium and dbcAMP on Sperm Motility in Mouse

Kim,  Moon-K; Oh,  Seung-H; Gye,  Myung-C; Yoon,  Hyun-S; Lee,  Joon-Y

Original Article

Korean Journal of Fertility and Sterility 1989;16(2):113-118.

Moon-K Kim¹, Seung-H Oh¹, Myung-C Gye¹, Hyun-S Yoon¹, Joon-Y Lee²

¹Department of Biology, College of Natural Sciences , Hanyang University
²Department of Biology, College of Natural Sciences, Chungbuk University

생쥐 정자의 운동성에 미치는 Calcium 과 dbcAMP 의 영향

김문규¹, 오승훈¹, 계명찬¹, 윤현수¹, 이준영²

¹한양대학교 자연과학대학 생물학과
²충북대학교 자연과학대학 생물학과

Abstract

In order to study the effects of $Ca^{++}$ and dbcAMP on sperm motility, cauda epididyal spermatozoa of mouse were incubated in the media containing the various concentrations of $Ca^{++}$ and dbcAMP, and Its motility were observed and analyzed. The rate(%) of the sperm with forward motility which may be fertile, reached at the equilibrium state in 30-60 min. after the beginning of incubation. $Ca^{++}$ requirement for the maintenance of sperm motility obviously appeared in 3hrs. after the beginning of incubation. In the medium containing 0.01mM $Ca^{++}$, the rate of sperm forward motility was highest(30.6-40.6%), whereas in 10mM $Ca^{++}$ medium, the rate was rather reduced because of a severe agglutination phenomenon. On the other hand, 1mM dbcAMP in the medium was significantly effective for the maintenance of sperm forward motility.