| Expression of Endometriosis Related Genes in the Shed Endometrial Tissues from Menstrual Blood. |
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Chan Woo Park, Jin Hyun Jun, Mi Kyoung Koong, In Ok Song |
1Division of Reproductive Endocrinology & Infertility, Department of Obstetrics and Gynecology, Kwandong University College of Medicine, Seoul, Korea.
2Laboratory of Reproductive Biology & Infertility Cheil General Hospital Kwandong University College of Medicine, Seoul, Korea. |
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| Abstract |
OBJECTIVE
This study was performed to investigate the expressions of endometriosis related genes in shed endometrial tissues from menstrual blood of patients with or without endometriosis. METHODS: The shed endometrial tissues were collected on 2nd or 3rd day of menstrual cycle with Wallace catheter in patients with endometriosis (n=16) and without endometriosis (n=26). The mRNA expressions of twelve kinds of endometriosis related genes were compared between two groups using semi-quantitative RT-PCR. RESULTS: The collected shed endometrium was confirmed by histological observation. Expressions of telomerase, c-kit and aromatase mRNA were not detected by RT-PCR in shed endometrial tissues. The mRNA expressions of apoptosis related genes (fas, fas ligand, bcl-2, bax), stem cell factor, estrogen receptor-alpha/alpha, endometriosis protein-I and secretory leukocyte protease inhibitor gene were similar between shed endometrial tissues with endometriosis and without endometriosis. CONCLUSION: We could not find the difference of mRNA expressions of tested endometriosis related genes between shed endometrial tissues with or without endometriosis by semi-quantitative RT-PCR analysis. It may be related to the dynamical changes of gene expressions in the endometrium with menstrual cycle. |
| Key Words:
Endometriosis; Shed endometrial tissues; mRNA expression; Semi-quantitative RT-PCR |
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