Korean J Reprod Med Search


Korean Journal of Reproductive Medicine 2007;34(4):275-283.
Published online December 1, 2007.
Expression of Endometriosis Related Genes in the Shed Endometrial Tissues from Menstrual Blood.
Chan Woo Park, Jin Hyun Jun, Mi Kyoung Koong, In Ok Song
1Division of Reproductive Endocrinology & Infertility, Department of Obstetrics and Gynecology, Kwandong University College of Medicine, Seoul, Korea.
2Laboratory of Reproductive Biology & Infertility Cheil General Hospital Kwandong University College of Medicine, Seoul, Korea.
This study was performed to investigate the expressions of endometriosis related genes in shed endometrial tissues from menstrual blood of patients with or without endometriosis. METHODS: The shed endometrial tissues were collected on 2nd or 3rd day of menstrual cycle with Wallace catheter in patients with endometriosis (n=16) and without endometriosis (n=26). The mRNA expressions of twelve kinds of endometriosis related genes were compared between two groups using semi-quantitative RT-PCR. RESULTS: The collected shed endometrium was confirmed by histological observation. Expressions of telomerase, c-kit and aromatase mRNA were not detected by RT-PCR in shed endometrial tissues. The mRNA expressions of apoptosis related genes (fas, fas ligand, bcl-2, bax), stem cell factor, estrogen receptor-alpha/alpha, endometriosis protein-I and secretory leukocyte protease inhibitor gene were similar between shed endometrial tissues with endometriosis and without endometriosis. CONCLUSION: We could not find the difference of mRNA expressions of tested endometriosis related genes between shed endometrial tissues with or without endometriosis by semi-quantitative RT-PCR analysis. It may be related to the dynamical changes of gene expressions in the endometrium with menstrual cycle.
Key Words: Endometriosis; Shed endometrial tissues; mRNA expression; Semi-quantitative RT-PCR


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