Korean J Reprod Med Search


Korean Journal of Reproductive Medicine 2009;36(3):163-174.
Published online September 1, 2009.
Expression of Immortalization-upregulated Proteins-2 (IMUP-2) in Placenta.
Su Yeon Jeon, Hyun Jung Lee, Hyun Min Jung, Jin Kyeoung Kim, Gi Jin Kim
1Department of Biomedical Science, CHA University, Seoul, Korea.
2Chabiotech Co., Ltd., CHA Stem Cell institute, Seoul, Korea.
OBJECTIVES: Members of the immortalization-upregulated protein (IMUP) family are nuclear proteins implicated in SV40-mediated immortalization and cellular proliferation, but the mechanisms by which their expression is regulated are still unknown in placenta. To investigate to expression and fuctions of IMUPs in placenta, we conducted to compare IMUPs expression in normal and preeclamptic placenta tissues and analyzed the function of IMUP-2 in HTR-8/SVneo trophoblast cells after IMUP-2 gene transfection. METHODS: The expression of IMUPs was analyzed in placental tissues from the following groups of patients (none underwent labor): 1) term normal placenta (n=15); 2) term with preeclamptic placeneta (n=15); and 3) pre-term with preeclamptic placenta (n=11) using semi-quantitative RT-PCR, RNA in situ hybiridization, immunohistochemistry, and Western blot. In order to evaluate the function of IMUP-2 in HTR-8/SVneo trophoblast cells, IMUP-2 plasmids were trnasfected into HTR-8/SVneo trophoblast cells for 24 hours. RESULTS: We observed that IMUPs are mainly expressed in the syncytiotrophoblasts and syncytial knot of placental villi. The expression of IMUP-1 was not differences between normal and preeclamptic placenta tissues. However, IMUP-2 expression was significantly higher in preterm preeclamptic placenta tissues than in normal placenta tissues without labor (p<0.001). Furthermore, we confirmed overexpression of IMUP-2 induced apoptosis in HTR-8/SVneo trophoblast cells through up-regulation of pro-apoptotic proteins. Conclusions: These results suggest that the expression of IMUP-2 is involved in placental development as well as increased IMUP-2 expression is associated with preeclampsia through the inducing of trophoblast apoptosis.
Key Words: Immortalization-upregulated proteins; Preeclampsia; Apoptosis; Hypoxia; Trophoblast


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