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Korean Journal of Reproductive Medicine 2010;37(3):207-216.
Published online September 1, 2010.
Role of HOXA Gene in Human Endometrial Decidualization.
Chang Se Lee, Dong Wook Park, Chan Woo Park, Tae Jin Kim
1Laboratory of Molecular Oncology, Cheil General Hospital, Kwandong University College of Medicine, Seoul, Korea.
2Laboratory of Reproductive Biology and Infertility, Cheil General Hospital, Kwandong University College of Medicine, Seoul, Korea.
3Department of Obstetrics and Gynecology, Cheil General Hospital, Kwandong University College of Medicine, Seoul, Korea.
Abstract
OBJECTIVE
This study was performed to clarify the role of HomeoboxA (HOXA) and its related signaling molecules in the decidualization of primary cultured endometrial cells. METHODS: Human endometrial tissues were obtained by curettage of hysterectomy specimens from patients with conditions other than endometrial diseases. Tissues were minced and digested with Trypsin-EDTA for 20 min, 37degrees C. Cells were cultured with DMEM/F12 medium in 37degrees C, 5% CO2 incubator for 24 hrs. Cells were treated with HOXA10 siRNA and added transforming growth factor (TGF)-beta1 (10 ng/mL) for 48 hrs to induces decidualization in vitro. Reverse transcription polymerase chain reaction analysis was accomplished to observe the expression of HOXA10, prolactin, cyclooxygenase (COX)-2, peroxisome proliferator-activated receptor (PPAR)-gamma, and wingless-type MMTV integration site family (Wnt). RESULTS: HOXA10 expression was increased (1.8 fold vs. non-treated control) in TGF-beta1 treated cells. Decidualization marker, prolactin, was significantly increased in TGF-beta1 treated cells compared with HOXA10 siRNA treated cells. Endometrial cell differentiation marker, COX-2 was down-regulated by HOXA10 siRNA even if cells were treated with TGF-beta1. Wnt4 was down-regulated by treated with HOXA10 siRNA, this expression patters was not changed by TGF-beta1. Expression of PPAR-gamma was down regulated by TGF-beta1 in regardless of HOXA10 siRNA treatment. CONCLUSION: TGF-beta1 which is induced by progesterone in endometrial epithelial cells may induces stromal cell decidualization via HOXA10 and Wnt signaling cascade.
Key Words: HOXA; Wnt; Decidualization; Endometrium


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