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Korean Journal of Fertility and Sterility 1998;25(1):71-76.
Published online January 1, 2001.
Developmental Capacity of Bovine Follicular Oocytes after Ultra-Rapid Freezing by Electron Microscope Grid I. Cryopreservation of Bovine Immature Oocytes.
E Y Kim, N H Kim, B K Yi, S H Yoon, S P Park, K S Chung, J H Lim
Abstract
SUMMARY This study was carried out to examine whether the developmental capacity of bovine immature oocytes frozen ultra-rapidly using electron microscope (EM) grids and EFS30 can be obtained. As freezing solution, we used EFS30 which consisted of 30% ethylene glycol, 0.5M sucrose, 18% ficoll and 10% FBS added in D-PBS. As criterion of oocyte viability, the rates of maturation, fertilization and embryonic development were determined. The results obtained in this experiment were summarized as follows: When ultra-rapidly frozen immature oocytes were thawed, 43.2% of them were survived. The rates of maturation (84.1%) and normal 2 pronuclei formation (57.5%) of frozen immature oocytes were not significantly different when compared to those of control (92.5, 65.0%). In addition, the rates of > or =2-cell (65.0%) and blastocyst formation (30.8%) of freezing group were not significantly different when compared to those of control (73.7, 35.7%). These results demonstrate that developmental capacity of frozen-thawed bovine immature oocytes can be successfully obtained when survived from the ultra-rapid freezing method using EM grid and EFS30.
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