| In Vitro/In Vivo Development of Mouse Oocytes Vitrified by EFS. |
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M K Kim, E Y Kim, B K Yi, S H Yoon, S P Park, K S Chung, J H Lim |
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| Abstract |
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SUMMARY This study was carried out to investigate in vitro/in vivo development of vitrified mouse oocytes. Mouse oocytes were vitrified using EFS30, 35 and 40 (30, 35 and 40% ethylene glycol, 18% ficoll and 0.5 M sucrose in M2medium). After being exposed or vitrified-thawed, oocytes of normal morphology were inseminated in vitro by 1-2x 106/m1 of epididymal sperm. The rates of fertilization, in vitro/in vivo development and cell number(inner cell mass and tropectoderm cell) of blastocysts in each treatment group were examined. The results obtained in these experiments were summarized as follows: The cleavage rates were obtained in EFS35 containing 35% ethylene glycol higher than in EFS30 and EFS40. The development rate of vitrified-thawed oocytes to two-cell stage after in vitro fertilization (S 1.1%) was significantly different compared to that of exposed to vitrification solution without cooling (60.0%) and control (68.2%) (p<0.05). However, there were no differences in the blastocyst formation from the cleaved embryos among groups (75.0, 73.3 and 80.0%). Also, the clean number of cells per blastocysts of vitrified group (92.5 +/- 2.9) was similar to that of the exposed (98.5 +/- 5.3) and control (100.9 +/- 4.8). In vivo development of the blastocysts derived from vitrified-thawed oocytes resulted in fetal development (50.7%) and implantation rates (80.0%) which are very similar to those of control (58.2, 78.2%). These results suggest that mouse oocytes could be cryopreserved using vitrification solution (EFS35) based on ethylene glycol. |
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