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Korean Journal of Fertility and Sterility 2000;27(3):253-259.
Published online January 1, 2001.
Preimplantation Development and Apoptosis of Mouse Embryos in the Medium Containing Extracellualr Matrix.
Byung Moon Kang, In Pyo Sohn, Byung Mok Chung, Kyoo Wan Choi, Myung Chan Gye
Abstract
OBJECTIVE
To verify the effect of Matrigel, a ECM complex from Engelbreth-Holm-Swarm(EHS) mouse sarcoma on the preimplantation development and apoptosis of mouse fertilized eggs. METHOD: Late Pronucleus stage eggs were cultured through the blastocyst stage in the presence of Matrigel (0.5%, v/v). Characteristics of apoptosis and cell number assessed by Hoecst staining and TUNEL labeling at the blastocyst stage, respectively. RESULTS: Morphological development, number of cells per embryo was significantly increased but rate and number of TUNEL positive nuclei of the embryo were decreased in the presence of Matrigel. CONCLUSION: This result suggested that at low concentration of Matrigel improves both viability and morphological development in the preimplantation mouse embryos.


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