Analysis of Human Spermatozoal Fertilizing Ability Using Zona -free Hamster Oocyte

Kim,  JM; Suh,  BH; Lee,  JH; Chung,  KS

Original Article

Korean Journal of Fertility and Sterility 1989;16(1):15-21.

¹Infertility Clinic, Department of Obstetrics and Gynecology, College of Medicine, Kyung Hee University
²College of Animal Husbandry, Kon-Kuk University

투명대 제거 햄스터난자를 이용한 인간정자 침투능에 관한 연구

김재명¹, 서병희¹, 이재현¹, 정길생²

¹경희대학교 의과대학 산부인과학교실(불임크리닉)
²건국대학교 축산대학

Abstract

An in vitro fertilization assay employing zona-free hamster embryos was used to investigate human spermatozoal fertilitzing ability. Yanaghimarchi et al.(1976) first introduced this cross species fertilzation technique, with its application as a diagnostic tool for male infertility. Human spermatozoa were preincubated for 3 to 4 hrs in B W W medium at concentration of $4{\times}10^6$ sperm/ml prior to the addition to zona-free hamster embryos. After 3 hrs, human sperm was evaluated for fertilizing potential by the presence of swelling or decondencing sperm head in the cytoplasm. The results of penetration rates for sperm were as follow : 1. The average penetration rate of a 7 fertile donor group was $47.8{\pm}27.67%$(Range 14.3-98.0%) 2. The average penetration rate of 12 infertile patients with normal semen analysis was $21.7{\pm}26.9%$(Range 0-38.8%) 3. The average penetration rate of 10 infertile patients with semen abnormalities was $6.1{\pm}8.1%$(Range 0-25%)