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Korean Journal of Fertility and Sterility 1987;14(2):93-100.
Studies on the Requirements of $Ca^{++}$ for Cell Division and $Ca^{++}$ Permeability of Plasma Membrane of Fast Dividing Mouse Embryo Cells.
In-Ha Bae1, Ji-Hye Park2
1Department. of Biology., College of Natural Science, Sungshin Women's University
2Department. of Zoology., Graduate School, Seoul National University
세포분열이 왕성한 생쥐 배세포에서 세포분열에 대한 $Ca^{++}$의 요구와 세포막투과성에 대한 연구
배인하1, 박지혜2
1성신여자대학교 자연과학대학 생물학과
2서울대학교 대학원 동물학과
Abstract
To determine the effect of calcium on the preimplantational development of mouse two-cell embryo, the various concentrations of calcium were added into the culture media and the rate of blastocyst formation was observed. Also, to examine the effect of trifluoperazine, an inhibitor of calmodulin which is involved in the several intracellular calcium functions, embryos were cultured for 48 hours at the various concentrations of this inhibitor. An additional 24 hour culture was done to examine the effect of this drug on the transformation from morula to blastocyst. The results are as following ; 1. About 1.71mM of extracellular calcium is adequate for blastocyst formation and the higher concentrations of calcium (3.43mM and 8.55mM) do not affect on the blastocyst formation and the degenerating rate. 2. Trifluoperazine $100{\mu}M$ presents the inhibitory effect on the blastocyst formation while $1{\mu}M$ and $10{\mu}M$ do not so. 3. After an additional 24 hour culture, there is transformation of morula to blastocyst and the degenerating rate of embryo is increased all together.
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