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Rapid freezing versus Cryotop vitrification of mouse two-cell embryos |
Namfon Inna, Usanee Sanmee, Ubol Saeng-anan, Waraporn Piromlertamorn, Teraporn Vutyavanich |
Clin Exp Reprod Med. 2018;45(3):110-115. Published online September 3, 2018 DOI: https://doi.org/10.5653/cerm.2018.45.3.110 |
Rapid freezing versus Cryotop vitrification of mouse two-cell embryos Rapid freezing of pronuclear, two-cell, eight-cell, morula, and blastocyst stage mouse embryos Closed-system solid surface vitrification versus slow programmable freezing of mouse 2-cell embryos The kitazato “closed” cryotop sc vitrification system performs comparably to its original “open” system. a study using unfertilized human eggs, mouse eggs, and mouse embryos Vitrification and ultra-rapid freezing of rat embryos Fast freezing of mouse embryos in classic straws with one equilibration step, using two vitrification media Effect of dimethyl sulfoxide and protein concentration on the viability of two-cell mouse embryos frozen with a rapid freezing technique Vitrification of mouse embryos at 2-cell, 4-cell and 8-cell stages by cryotop method Comparison of two-step vitrification versus controlled freezing on survival of in vitro produced cattle embryos Highly efficient vitrification for cryopreservation of human oocytes and embryos: The Cryotop method |