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Efficient isolation of sperm with high DNA integrity and stable chromatin packaging by a combination of density-gradient centrifugation and magnetic-activated cell sorting |
Hee-Jun Chi, Su-Jin Kwak, Seok-Gi Kim, Youn-Young Kim, Ji-Young Park, Chang-Seok Yoo, Il-Hae Park, Hong-Gil Sun, Jae-Won Kim, Kyeong-Ho Lee |
Clin Exp Reprod Med. 2016;43(4):199-206. Published online December 26, 2016 DOI: https://doi.org/10.5653/cerm.2016.43.4.199 |
Efficient isolation of sperm with high DNA integrity and stable chromatin packaging by a combination of density-gradient centrifugation and magnetic-activated cell sorting Semen processing by density gradient centrifugation is useful in selecting sperm with higher double-strand DNA integrity Chromatin intact human sperm recovery is higher following glass wool column filtration as compared with density gradient centrifugation The comparative effect of magnetic activated cell sorting, density gradient centrifugation and swim up on assisted reproduction outcomes, sperm DNA fragmentation, and aneuploidy: A systematic review and meta-analysis MACS (magnetic activated cell sorting) before or after density gradient centrifugation for seminal cryopreservation Isolation of glial cell-enriched and -depleted populations from mouse cerebellum by density gradient centrifugation and electronic cell sorting Density gradient centrifugation before or after magnetic-activated cell sorting: which technique is more useful for clinical sperm selection? Is Magnetic-Activated Cell Sorting an Efficient Technique in Reducing Human Sperm DNA Fragmentation? The Effect of Density Gradient Centrifugation and Magnetic-Activated Cell Sorting with the Combination of Both on Bovine Spermatozoa Kinematics, Viability and Acrosome Status Low-stress Microfluidic Density-gradient Centrifugation for Blood Cell Sorting |