In Vitro Developments of Porcine Embryos Produced by In Vitro Fertilization and Parthenogenetic Activation.

Uhm,  S J; Kim,  N H; Kim,  E Y; Kim,  M K; Cha,  S K; Yoon,  S H; Park,  S P; Chung,  K S; Lim,  J H

Original Article

Korean Journal of Fertility and Sterility 1997;24(1):43-49.
Published online January 1, 2001.

In Vitro Developments of Porcine Embryos Produced by In Vitro Fertilization and Parthenogenetic Activation.

S J Uhm, N H Kim, E Y Kim, M K Kim, S K Cha, S H Yoon, S P Park, K S Chung, J H Lim

Abstract

The objective of this study is to determine developmental pattern and cell allocation to the ICM and TE in haploid and diploid of embryos following parthenogenetic activation and in vitro fertilization. The incidence of development to blastocyst was lower in the combined treatments of ethanol stimulation and cytochalasin B as compared to the control. However, the combined ethanol stimulation and cytochalasin B treatment (diploid) accelerated development to the blastocyst as compared to the ethanol treatment alone (haploid). Significantly reduction in the average number of total cells and ICM was observed in the parthenotes alone as compared to fetilized embryos, but those of combined ethanol stimulation and cytochalasin B treatment embryos were significantly increased as compared to ethanol alone embryos. These results suggested that the ploidy affects preimplantation developmental patters and cell allocation to the ICM and TE in the porcine.