| Studies of Changes of Ca2+-channels Distribution in the Activated Mouse Ova. |
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Yeon Soo Chang, In Ha Bae |
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Department of Biology, College of Natural Sciences, Sungshin Women's University, Seoul, Korea. |
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| Abstract |
OBJECTIVE
In muscle and neuronal cells, calcium channels have been classified by electrophysiological and pharmacological properties into (1) voltage-dependent Ca2+-channel(1) P/Q-type Ca2+-channel (2) N-type Ca2+-channel R(3) L-type Ca2+-channel (4) T-type Ca2+-channel (5)R-type Ca2+-channel. The present study was done in order to investigate whether there is any difference in Ca2+-channel distribution between activated and normally fertilized embryos. METHODS: The immunocytochemical method was used to identify the existence of voltage-dependent Ca2+-channels in parthenogenetically activated 2-cell embryos by ethanol and SrCl2 treatment. These 2-cell embryos were obtained by exposure to 6% ethanol for 6 min and to 10 mM SrCl2 for 2h. RESULTS: P/Q-type Ca2+ channels and L-type Ca2+-channels have been identified. Whereas, three type of Ca2+-channel P/Q-type, N-type, L-type have been identified in 2-cell embryos fertilized in vivo. CONCLUSION: Activation by ethanol was faster than those by SrCl2. However, there was difference in DAB staining of the embryos between ethanol and SrCl2 treatment (87.7% and 54.1%). Intensity of staining was also different between ethanol- and SrCl2-treated group. However, it has not been known why there was some difference in DAB staining and staining intensity in the present study. |
| Key Words:
Parthenogenesis; Mouse oocyte; Ca2+-channel |
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