| Study on Pregnancy and Viability of Frozen-Thawed Human Embryos by Cryopreservation : DMSO as Cryoprotectant |
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Ho-Joon Lee1, Seung-Jae Lee1, Sung-Il Roh1, Hye-Ran Paik1, Moon-Kyoo Kim2 |
1IVF Research Laboratory, Department of Obstetrics and Gymecology, Cheil General Hospital
2Department of Biology, College of Natural Sciences, Hanyang University |
| 동결보존에 의한 인간배아의 생존률과 임신에 관한 연구 |
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이호준1, 이승재1, 노성일1, 백혜란1, 김문규2 |
1제일병원 산부인과 체외수정 연구실
2한양대학교 자연과학대학 생물학과 |
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| Abstract |
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This study was done to verify factors affecting viability after cryopreservation and pregnancy rate after frozen-thawed embryo transfer into uterus. Embryos were cryopreserved slow freezing and slow thawing and used DMSO as cryoprotectant. The results were to follows. 1. Viability of frozen-thawed embryos were 75.5% (94/105), which compared with viability of embryos according to cell stage, $2{\sim}5$ cell was 68.4% and $6{\sim}16$ cell 80.4% were significant differences (p<0.05). 2. No significant difference in duration of cryopreservation on effects affecting pregnancy rate was observed. 3. Number of embryo transfered into uterus was significant differences (p<0.05). 4. Four pregnancies resulted following replacement of 35 frozen-thawed. |
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