| The Relationship Between the Pyknosis of Granulosa Cell and the Change of Steroid Concentration in Porcine Ovarian Follicles |
|
Chang-Joo Lee, Yong-Dal Yoon, Jong-Heup Kim, Moon-Kyoo Kim |
|
Department of Biology, College of Natural Sciences, Hanyang University |
| 돼지 난소내 과립세포의 염색질이상응축과 여포액내 스테로이드호르몬의 농도 변화와의 상관관계 |
|
이창주, 윤용달, 김종흡, 김문규 |
|
한양대학교 자연과학대학 생물학과 |
|
|
| Abstract |
|
In order to study the follicular atretic mechanism in mammalian ovary, the relationship between pyknotic index (PI) of granulosa cells and the steroid concentrations in the follicular fluid of atretic follicle was investigated. Follicles were isolated from porcine ovary according to their sizes and the reproductive phases. Steroid concentrations were quantified by high-performance liquid chromatography. As PI increased, the concentration of progesterone was significantly increased(p<0.05), whereas testosterone and estradiol showed no significant changes in their concentration. As follicular size was increased, PI of follicular GC in the luteal phase was increased significantly(p<0.05) and the molar ratio of progesterone to testosterone was increased in the follicles of follicular phase. It can be concluded that progesterone accumulated in the follicular fluid as atresia of the follicles was progressed, and that PI of granulosa cells could be used as one of convenient and pratical criteria for the identification of follicular atresia. |
|
PDF Links
Full text via DOI
Download Citation
